微信公众号

请长按图片识别二维码

模型资源

首页 模型资源 重度免疫缺陷模型 SDRG

Immunodeficient rats represent powerful tools to investigate the pathobiology of B cell disorders such as leukemias and lymphomas, as well as lymphoid compartment development.

They are valid alternatives to immunodeficient mice since, e.g., the liquid nature of malignancies like B cell lymphoma make downstream analysis quite challenging due to small blood volumes of mice.

Compared to the traditional immunodeficient nude rats, which lack T cells but maintain a normal repertoire of all other immune cells, including B and NK cells, the SDRG model is a double Knockout for Rag1 and IL-2Rγ genes (Rag1-/- Il2rγ-/-), resulting in a B, T and NK cell deficiency.

‍

Applications

The SDRG rat represents a valid preclinical model to study:

  • Lymphoid compartment development
  • B cell malignancies (e.g., leukemias, lymphomas)
  • Xenografts
  • Inflammatory disorders
  • Infectious diseases

SDRG rats display no T, B and NK and NKT cells

Hemocytometer and flow cytometry count of T, B, NK, NKT and myeloid cells in SDRG and wild-type Sprague Dawley rats. SDRG rats display a significantly reduced number of T and B cells (upper graphics) and NK cells (lower graphics) compared to wild-type Sprague Dawley rats. Flow cytometry analyses of T, B, NK, NKT and myeloid cells (CD3+ TCRαβ+, CD3- CD45R+, CD3- TCRαβ- CD335+ CD161+, CD3+ TCRαβ- CD335+ CD161+, CD3- CD45R- CD161- respectively) in blood and spleen.

 

更多详细数据请联系我们：联系我们 - 基锘威 - genOway (my3w.com)

Case studies

  1. New insights to manage painful neuroma: Study of a nerve graft in rats
  2. Tips for choosing the optimal preclinical model: focus on atherosclerosis and LDLR
  3. The importance of genetic design when choosing your preclinical model: focus on preclinical models for cardiovascular disorders — a case study on BMPR2 
  4. When rat models could provide optimized translatability: the case of CD89

Commentaries

  1. Tumor grafts in preclinical research: Models, models, overall, who is the fittest of them all?
  2. Back to the rat! 

Scientific articles

  1. Early lysosome defects precede neurodegeneration with amyloid-β and tau aggregation in NHE6-null rat brain\
  2. The Lrat-/- Rat: CRISPR/Cas9 Construction and Phenotyping of a New Animal Model for Retinitis Pigmentosa
  3. Long Acellular Nerve Allografts Cap Transected Nerve to Arrest Axon Regeneration and Alter Upstream Gene Expression in a Rat Neuroma Mode
  4. Comparison of Myelin-Associated Glycoprotein With Vincristine for Facial Nerve Inhibition After Bilateral Axotomy in a Transgenic Thy1-Gfp Rat Model
  5. Assessment of fear and anxiety associated behaviors, physiology and neural circuits in rats with reduced serotonin transporter (SERT) levels
  6. Constitutive serotonin transporter reduction resembles maternal separation with regard to stress-related gene expression
  7. 5-HT3 receptor signaling in serotonin transporter knockout rats: a female sex specific animal model of visceral hypersensitivity.
  8. Generation of immunodeficient rats with Rag1 and Il2rg gene deletions and human tissue grafting models.
  9. Acute and subchronic antinociceptive effects of nociceptin/orphanin FQ receptor agonists infused by intrathecal route in rats.
  10. Serotonin Reuptake Transporter Deficiency Modulates the Acute Thermoregulatory and Locomotor Activity Response to 3,4-({+/-})-Methylenedioxymethamphetamine, and Attenuates Depletions in Serotonin Levels in SERT-KO Rats
  11. Limited regeneration in long acellular nerve allografts is associated with increased Schwann cell senescence.
  12. Nitric oxide regulates cardiac intracellular Na+ and Ca2+ by modulating Na/K ATPase via PKCε and phospholemman-dependent mechanism.
  13. Visceral hypersensitivity in female but not in male serotonin transporter knockout rats.
  14. Generation of Rag1-knockout immunodeficient rats and mice using engineered meganucleases.
  15. Mesenchymal stem cell therapy prevents IFTA in a rat kidney allograft model
  16. Geldanamycin accelerated peripheral nerve regeneration in comparison to FK-506 in vivo.
  17. A transgenic rat expressing green fluorescent protein (GFP) in peripheral nerves provides a new hindlimb model for the study of nerve injury and regeneration
  18. Effects of Human Alpha-Synuclein A53T-A30P Mutations on SVZ and Local Olfactory Bulb Cell Proliferation in a Transgenic Rat Model of Parkinson Disease
  19. Lack of the serotonin transporter (SERT) reduces the ability of 5-hydroxytryptamine to lower blood pressure.
  20. Comparison of the function of the serotonin transporter in the vasculature of male and female rats
  21. Pharmacological blockade or genetic knockout of the NOP receptor potentiates the rewarding effect of morphine in rats.
  22. Nociceptin/orphanin FQ receptor knockout rats: in vitro and in vivo studies.
  23. Serotonin transporter interacts with the PDGFβ receptor in PDGF-BB-induced signaling and mitogenesis in pulmonary artery smooth muscle cells
  24. A New Model for Facial Nerve Research: the novel transgenic Thy1-GFP rat
  25. Identification of genetic modifiers of behavorial phenotypes in serotonin transporter knockout rats
  26. Body distribution of infused serotonin in rats.