The CT26-hEpCAM-LZ clonal cell line expresses high levels of human EpCAM, and forms solid tumors in vivo. An optimized version of a luciferase-ZsGreen (LZ) fusion protein is also stably expressed for in vivo imaging and ex vivo tracking.
Expression analysis by flow cytometry. Human EpCAM expression assessed in CT26.WT parental cell line (left), human control cell line (center), and CT26-hEpCAM cell line (right).
Wild-type Balb/c mice were injected (s.c.) with 1x106 CT26-LZ (left) or CT26-hEpCAM-LZ (right) cells. Tumor growth was measured every 2 to 3 days. Tumor size (mm3) = (width2 x length)/2.
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