微信公众号

请长按图片识别二维码

模型资源

首页 模型资源 细胞资源 MC38-hPD-L1-hHER2-LZ

Design of the MC38-hPD‑L1-hHER2-LZ cell line

The MC38 cell line was derived from C57BL/6 murine colon adenocarcinoma cells. They form solid tumors upon subcutaneous injection in immunocompetent mice.

We generated a MC38-hPD-L1-hHER2-LZ clonal cell line expressing high levels of human PD-L1 (CD274 NCBI GeneID: 29126) and human HER2 (ERBB2 NCBI GeneID: 2064), and an optimized dual fusion reporter luciferase-ZsGreen (LZ). These cells are invalidated for murine Pd-l1 (Cd274, NCBI Gene ID: 60533). MC38-hPD-L1-hHER2-LZ cells form solid tumors in vivo. Isogenic wild-type (WT) and luciferase-ZsGreen (LZ) control clones are available.

‍

MC38-hPD-L1-hHER2-LZ features

  • Express high levels of full human PD-L1 and HER2
  • Express a luciferase-ZsGreen (LZ) fusion reporter
  • Form solid tumors upon subcutaneous injection in fully immunocompetent mice
  • Injected cells can be followed in vivo and ex vivo with our LZ reporter

  • MC38-hPD-L1-hHER2-LZ cells homogeneously express human PD‑L1 and HER2 in vitro

    Expression analysis by flow cytometry. Human PD-L1 and HER2 expression assessed in MC38 parental cell line (top left), human control cell line – RKO (bottom left) and BT474 (bottom right), and MC38-hPD-L1-hHER2-LZ cell line (top right). Human HER2 was detected using Trastuzumab antibody.

     

  • MC38-hPD-L1-hHER2-LZ cells form tumors in vivo

    C57/Bl6 mice were injected (s.c.) with 2x106 MC38-hPD-L1-LZ cells (left) or MC38-hPD-L1-hHER2-LZ cells (right). Tumor growth was measured every 2 to 3 days. Tumor size (mm3) = (width² x length)/2.

更多详细数据请联系我们：联系我们 - 基锘威 - genOway (my3w.com)

Commentaries

  1. Development of a new hSIRPα-specific radiotracer for non-invasive PET imaging using genOway’s double humanized hCD47/hSIRPα mouse and MC38-hPD-L1-hCD47-LZ cell line
  2. CCX559 is a potent, orally-administered small molecule PD-L1 inhibitor that induces anti-tumor immunity
  3. A new promising family of small molecules regulating the PD-L1/PD-1 signaling pathway
  4. Improving in vitro models: the coming of age of organoids
  5. iPS technology: Four factors that will change the world… some day

Scientific articles

  1. Two birds with one stone: human SIRPα nanobodies for functional modulation and in vivo imaging of myeloid cells
  2. CCX559 is a potent, orally-administered small molecule PD-L1 inhibitor that induces anti-tumor immunity
  3. ERα-36 regulates progesterone receptor activity in breast cancer
  4. T-cells expressing a chimeric-PD1-Dap10-CD3zeta receptor reduce tumour burden in multiple murine syngeneic models of solid cancer
  5. Tumor Vessel Normalization, Immunostimulatory Reprogramming, and Improved Survival in Glioblastoma with Combined Inhibition of PD-1, Angiopoietin-2, and VEGF
  6. SDHA gain-of-function engages inflammatory mitochondrial retrograde signaling via KEAP1-Nrf2